- In the cyanmethemoglobin method, hemoglobin is converted into stable cyanmethemoglobin by reacting with potassium ferricyanide and potassium cyanide.
- This form is stable and measurable spectrophotometrically, allowing accurate determination of total hemoglobin concentration.
- Other forms like methemoglobin or carboxyhemoglobin are not stable or suitable for this method.
- The use of cyanmethemoglobin standardizes the assay, ensuring reliability and reproducibility.