- The definitive diagnosis of intestinal amoebiasis relies on the direct identification of Entamoeba histolytica trophozoites in stool samples.
- Microscopic detection of these trophozoites confirms active infection and differentiates it from nonpathogenic amoebae.
- Other tests such as eosinophilia, stool occult blood, or elevated serum alkaline phosphatase lack specificity and are not diagnostic.
- Therefore, stool microscopy showing trophozoites is the gold standard for diagnosis.

Reference: Davidson’s Principles and Practice of Medicine, 24th Edition, Chapter: Gastrointestinal infections, Page 608.