5.25% sodium hypochlorite is the most effective for immediate chairside sterilization of gutta-percha points because it rapidly kills bacteria, spores, and fungi within seconds to minutes without compromising the physical integrity of the gutta-percha. Unlike glutaraldehyde, which requires a longer contact time, sodium hypochlorite provides fast and reliable disinfection suitable for clinical use. It also penetrates the surface effectively, ensuring thorough microbial elimination before obturation. This makes it the preferred choice for instant chairside sterilization in endodontic practice.

Reference: Cohen’s Pathways of the Pulp, 12th Edition.