The most suitable method for sterilizing heat-sensitive liquids like culture media is filtration because it physically removes microorganisms without the need for heat. Autoclaving and dry heat involve high temperatures that can denature essential nutrients and growth factors in the media. Filtration using membrane filters (0.22 µm pore size) effectively excludes bacteria and fungi while preserving the liquid’s integrity. Radiation
Reference: Medical Microbiology, Murray et al., 9th Edition.